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Quantitative Assessment of Low-Dose Photodynamic Therapy Effects on Diabetic Wound Healing Using Raman Spectroscopy
Hala Zuhayri1, Alice A Samarinova1, Alexey V Borisov1
1Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Lenin Ave. 36, Tomsk 634050, Russia.
Abstract:
One of challenges that faces diabetes is the wound healing process. The delayed diabetic wound healing is caused by a complicated molecular mechanism involving numerous physiological variables. Low-dose photodynamic therapy (LDPDT) provides excellent results in rejuvenation and wound healing. In this study, the LDPDT effect on diabetic wounds in mice was studied using two photosensitizers, 5-aminolevulinic acid and methylene blue, and two laser dose expositions of 1 J/cm2 and 4 J/cm2 by Raman spectroscopy (RS). The latter was used as a noninvasive method, providing specific information about tissue state based on the fundamental vibrational modes of its molecular components. RS allows high spatial resolution acquisition of biochemical and structural information through the generation of point spectra or spectral images. An approach to in vivo quantitative assessment of diabetic wound healing state was developed. This approach is based on an application of the principal component analysis combined with the Mahalanobis metrics to skin Raman spectra, in particular, intensities of the amide I and CH2 bands.
Insights
Low-dose photodynamic therapy (LDPDT) effectively promotes diabetic wound healing in mice. Raman spectroscopy noninvasively assessed healing by analyzing molecular changes in the skin.
Area of Science:
- Biomedical Engineering
- Photomedicine
- Dermatology
Background:
- Diabetic wound healing is a significant clinical challenge due to complex molecular mechanisms.
- Delayed healing in diabetes involves numerous physiological variables.
- Low-dose photodynamic therapy (LDPDT) shows promise for tissue rejuvenation and wound healing.
Purpose of the Study:
- To investigate the efficacy of LDPDT on diabetic wound healing in a mouse model.
- To evaluate the impact of different photosensitizers (5-aminolevulinic acid, methylene blue) and laser doses (1 J/cm², 4 J/cm²).
- To develop a noninvasive method for quantitative assessment of diabetic wound healing using Raman spectroscopy.
Main Methods:
- Utilized LDPDT with two photosensitizers and two laser doses on diabetic mouse wounds.
- Employed Raman spectroscopy (RS) as a noninvasive technique to analyze tissue molecular composition.
- Developed a quantitative assessment approach using principal component analysis and Mahalanobis metrics on skin Raman spectra (Amide I and CH₂ bands).
Main Results:
- LDPDT demonstrated positive effects on diabetic wound healing.
- Raman spectroscopy provided specific molecular and structural information about the tissue state.
- The developed RS-based approach enabled in vivo quantitative assessment of wound healing progression.
Conclusions:
- LDPDT is a promising therapeutic strategy for accelerating diabetic wound healing.
- Raman spectroscopy offers a valuable noninvasive tool for monitoring wound healing.
- Quantitative analysis of Raman spectra can effectively assess the in vivo state of diabetic wound healing.
