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Related Experiment Video

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Chlorambucil-Loaded Graphene-Oxide-Based Nano-Vesicles for Cancer Therapy.

Surabhi Kumari1, Anuj Nehra1,2, Kshitij Gupta3

  • 1Bio-Nanotechnology Research Laboratory, Biophysics Unit, College of Basic Science & Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263145, Uttarakhand, India.

Pharmaceutics
|February 25, 2023
PubMed
Summary
This summary is machine-generated.

This study developed biocompatible graphene oxide (GO) nano-vesicles for targeted cancer drug delivery. These nano-vesicles show high chlorambucil (CHL) loading and controlled release, with reduced toxicity for biomedical applications.

Keywords:
cancerchlorambucildrug loadinggraphene-oxidein-vitronano-vesicles

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery Systems

Background:

  • Graphene oxide (GO) offers a promising platform for drug delivery due to its unique properties.
  • Targeted delivery of chemotherapeutic agents like chlorambucil (CHL) can improve efficacy and reduce side effects.
  • Functionalization of GO with targeting ligands enhances cellular uptake and specificity.

Purpose of the Study:

  • To design and characterize biocompatible nano-vesicles using folic acid (FA)-conjugated graphene oxide (GO) for chlorambucil (CHL) drug delivery.
  • To investigate the drug loading efficiency and in vitro release profile of CHL from the functionalized GO nano-vesicles at different pH values.
  • To evaluate the biotoxicity of the developed nano-vesicles against a human cervical adenocarcinoma cell line.

Main Methods:

  • Sulfonation and folic acid (FA) conjugation of graphene oxide (GO) sheets.
  • Drug loading of chlorambucil (CHL) onto functionalized GO via π-π stacking and hydrophobic interactions.
  • In vitro drug release studies at pH 7.4 and 5.5.
  • Biotoxicity assessment using cell viability assays (IC50 determination).

Main Results:

  • FA-conjugated GO sheets (CHL-GO) exhibited an average particle size of 300 nm, 58% absorption, and 77% loading efficiency.
  • Drug release was pH-dependent, with faster release observed at acidic pH (5.5) compared to physiological pH (7.4).
  • CHL-loaded GO showed significantly lower cytotoxicity (IC50 = 18 μM) compared to CHL-free GO (IC50 = 8 μM) against the cancer cell line.

Conclusions:

  • Folic acid-conjugated graphene oxide nano-vesicles demonstrate high drug-loading capacity and controlled release of chlorambucil.
  • The developed nano-vesicles exhibit favorable biotoxicity profiles, indicating their potential for targeted cancer therapy.
  • These findings highlight the significant potential of CHL-GO nano-vesicles in advanced biomedical applications.