Characterization and Therapeutic Applications of Biosynthesized Silver Nanoparticles Using Cassia auriculate Flower
Nadana Sabapathi1, Srinivasan Ramalingam2, Kandasamy Nagarajan Aruljothi3
1Guangdong Key Laboratory for Genome Stability and Disease Prevention, School of Medicine, Shenzhen University, Shenzhen 518060, China.
Abstract:
The current study analyzes the biosynthesis of silver nanoparticles using the Cassia auriculate flower extract as the reducing and stabilizing agent. The Cassia auriculate- silver nanoparticles (Ca-AgNPs) obtained are characterized by UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analysis. The results of the spectral characterization have revealed that the surface Plasmon resonance band observed at 448 nm confirms the formation of AgNPs. TEM analysis of the Ca-AgNPs was a predominately spherical shape with a size assortment of 30 to 80 nm and an angular size of 50 nm. The well-analyzed Ca-AgNPs were used in various biological assays, including healthcare analysis of antimicrobial, antioxidant (DPPH), and cytotoxic investigations. Ca-AgNPs showed efficient free radical scavenging activity and showed excellent antimicrobial activity against to pathogenic strains. The occurrence of Ca-AgNPs lead to reduced Live/Dead ratio of bacteria (from 36.97 ± 1.35 to 9.43 ± 0.27) but improved the accumulation of bacterial clusters. The cytotoxicity of Ca-AgNPs was carried out by MTT assay against MCF-7 breast cancer cells and a moderate cytotoxic. The approach of flower extract-mediated synthesis is a cost-efficient, eco-friendly, and easy alternative to conventional methods of silver nanoparticle synthesis.
