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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Aug 8, 2025

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Gold Nanomaterials-Implemented CRISPR-Cas Systems for Biosensing.

Ruijie Fu1, Yunlei Xianyu1,2,3

  • 1State Key Laboratory of Fluid Power and Mechatronic Systems, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, P. R. China.

Small (Weinheim an Der Bergstrasse, Germany)
|February 25, 2023
PubMed
Summary

CRISPR-Cas biosensors integrated with gold nanomaterials offer precise detection of DNA and RNA. This review highlights advances in these biosensing systems, focusing on mechanisms and performance for point-of-care applications.

Keywords:
biosensingclustered regularly interspaced short palindromic repeats (CRISPR)-Cas systemsgold nanomaterialssignal output

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems are valuable for biosensing due to precise nucleic acid targeting and cleavage.
  • Gold nanomaterials offer excellent optical properties and surface functionalization for biosensor development.

Purpose of the Study:

  • To review recent advancements in CRISPR-Cas-based biosensors utilizing gold nanomaterials.
  • To discuss the working mechanisms, performance, and applications of these integrated biosensing systems.

Main Methods:

  • Review of literature on CRISPR-Cas systems (Cas9, Cas12a, Cas13a) combined with gold nanomaterials (nanoparticles, nanorods, nanostars).
  • Analysis of biosensing strategies for DNA and RNA detection.
  • Evaluation of signal generation through nucleic acid degradation and nanomaterial interactions.

Main Results:

  • CRISPR-Cas systems coupled with gold nanomaterials enable sensitive and specific detection of various targets.
  • Integration enhances signal amplification and allows for diverse readout methods.
  • These biosensors show promise for point-of-care diagnostics.

Conclusions:

  • Gold nanomaterial-enhanced CRISPR-Cas biosensors represent a powerful platform for nucleic acid detection.
  • Further research is needed to address challenges and optimize performance for widespread application.
  • These systems offer versatile solutions for diagnostics and molecular detection.