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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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CircDHRS3 inhibits prostate cancer cell proliferation and metastasis through the circDHRS3/miR-421/MEIS2 axis

Xiyu Dai1,2, Xinan Chen1,2, Wensun Chen1,2

  • 1Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

Epigenetics
|February 25, 2023
PubMed

Insights

Circular RNA DHRS3 (circDHRS3) is underexpressed in prostate cancer. Overexpressing circDHRS3 inhibits cancer growth and metastasis by targeting miR-421 and MEIS2, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer is a leading global cancer in men.
  • Circular RNAs (circRNAs) are increasingly recognized for their role in cancer malignancy.
  • Understanding circRNA function is crucial for developing new prostate cancer therapies.

Purpose of the Study:

  • To investigate the role and mechanism of circDHRS3 in prostate cancer.
  • To explore circDHRS3 as a potential biomarker and therapeutic target.

Main Methods:

  • circRNA sequencing and quantitative real-time PCR (qRT-PCR) for expression analysis.
  • In vitro assays (CCK-8, colony formation, wound-healing) and in vivo xenograft models in BALB/c Nude mice.
  • Molecular mechanism studies including RIP assay, FISH, RNA pulldown, dual-luciferase, and rescue assays.

Main Results:

  • circDHRS3 expression was found to be low in prostate cancer tissues.
  • Overexpression of circDHRS3 suppressed prostate cancer cell proliferation and migration in vitro.
  • circDHRS3 targets miR-421, and the circDHRS3/miR-421/MEIS2 axis regulates cancer cell proliferation and metastasis.
  • In vivo studies confirmed circDHRS3 inhibits lung and bone metastasis of prostate cancer cells.

Conclusions:

  • circDHRS3 acts as a tumor suppressor in prostate cancer.
  • The circDHRS3/miR-421/MEIS2 pathway is a key mechanism driving prostate cancer progression.
  • circDHRS3 holds promise as a diagnostic biomarker and therapeutic target for advanced prostate cancer.
Keywords:
MEIS2circDHRS3circRNAprostate cancer

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