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Updated: Aug 8, 2025

Biophysical Characterization of Flagellar Motor Functions
Published on: January 18, 2017
Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo
Hiroyuki Terashima1, Michio Homma2, Seiji Kojima2
1Department of Bacteriology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan. terashima.hiroyuki@nagasaki-u.ac.jp.
Abstract:
The bacterial flagellum employs a rotary motor embedded on the cell surface. The motor consists of the stator and rotor elements and is driven by ion influx (typically H+ or Na+) through an ion channel of the stator. Ion influx induces conformational changes in the stator, followed by changes in the interactions between the stator and rotor. The driving force to rotate the flagellum is thought to be generated by changing the stator-rotor interactions. In this chapter, we describe two methods for investigating the interactions between the stator and rotor: site-directed in vivo photo-crosslinking and site-directed in vivo cysteine disulfide crosslinking.
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