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Updated: Aug 8, 2025

Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa
Published on: April 8, 2016
Twitching Motility Assays of Lysobacter enzymogenes OH11 Under a Light Microscope
Bingxin Wang1, Xiaolong Shao1, Guoliang Qian2
1College of Plant Protection, State Key Laboratory of Biological Interactions and Crop Health, Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Nanjing, People's Republic of China.
Abstract:
Bacterial twitching motility is a peculiar way of adherence and surface translocation on moist solid or semisolid surfaces. Although the twitching motility has been detected in various flagellated bacteria, such as Pseudomonas aeruginosa, it has been rarely detected in flagella-less bacteria like Lysobacter enzymogenes, a natural predator of filamentous fungi. Here, by using a strain OH11 of L. enzymogenes as a model system, we describe a convenient method for observing the twitching motility, with fewer steps and better repetition than conventional methods. This new method provides important technical support for the motile study of Lysobacter.

