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Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
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Motility Assays of Mycoplasma mobile Under Light Microscopy
Taishi Kasai1, Makoto Miyata2,3,4,5
1College of Science, Department of Life Science, Rikkyo University, Tokyo, Japan. t.kasai@rikkyo.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2023
Abstract:
Mycoplasma mobile forms a membrane protrusion at a pole as an organelle. M. mobile cells bind to solid surfaces and glide in the direction of the protrusion. In gliding motility, M. mobile cells catch, pull and release sialylated oligosaccharides on host cells. The observation of Mycoplasma species under light microscopy is useful for the analysis of adhesion ability and the motility mechanism.

