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Updated: Aug 8, 2025

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays
Published on: April 7, 2015
Swimming Motility Assays of Spiroplasma
1Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Tokyo, Japan. dice-k@uec.ac.jp.
Abstract:
Spiroplasma swim in liquids without the use of the bacterial flagella. This small helical bacterium propels itself by generating kinks that travel down the cell body. The kink translation is unidirectional, from the leading pole to the lagging pole, during cell swimming in viscous environments. This protocol describes a swimming motility assay of Spiroplasma eriocheiris for visualizing kink translations of the absolute handedness of the body helix with optical microscopy.
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