Related Experiment Video
Updated: Jul 29, 2026
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Quantitative proteomic analysis of heifer serum at the peri-implantation period using two complementary methods
Jimena Inés Ruiz Álvarez1, Juan Manuel Teijeiro2, Caroline Evans3
1Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Context:
Systemic events resulting from mother-embryo relation at the peri-implantation period may result in specific changes to the protein composition of serum and thus provide a source of biomarkers for early detection of pregnancy.
Aims:
We set out to use two different quantitative proteomic approaches to test this hypothesis by comparing heifer serum at the peri-implantation period to that from cycling heifers.
Methods:
Two-dimensional electrophoresis (2-DE) and isobaric tags were used for relative and absolute quantitation (iTRAQ) of proteins.
Key Results:
These methods yielded complementary data indicating biomarker candidate proteins.
Conclusions:
Different proteomic methods provide different and complementary information that needs to be analysed in order to consider proteins as potential biomarkers.
Implications:
In order to characterise the proteome under specific conditions, the use of complementary techniques is advisable.
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