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Updated: Aug 8, 2025

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Phase Heterogeneity in Cholesterol-Containing Ternary Phospholipid Lamellar Phases
Deborah L Gater1, Keontré I Hughes2,3, Vivian Stojanoff4
1University College London, London WC1E 6BT, U.K.
None:
Pseudo-ternary mixtures of lamellar phase phospholipids (DPPC and brain sphingomyelin with cholesterol) were studied below T m while comparing the influence of cholesterol content, temperature, and the presence of small quantities of vitamin D binding protein (DBP) or vitamin D receptor (VDR). The measurements, conducted by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR), cover a range of cholesterol concentrations (20% mol. wt to 40% mol. wt.) and physiologically relevant temperature range (294-314 K). In addition to rich intraphase behavior, data and modeling are used to approximate the lipids' headgroup location variations under the abovementioned experimental conditions.
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