Essential terminology and considerations for validation of non-targeted methods
Kapil Nichani1,2, Steffen Uhlig3, Manfred Stoyke4
1QuoData GmbH, Prellerstr. 14, 01309 Dresden, Germany.
Food Chemistry: X
|February 27, 2023
Summary
Non-targeted methods (NTMs) analyze all sample components, unlike traditional approaches. This paper clarifies NTM terminology and offers a roadmap for validating these powerful analytical techniques in food and feed testing.
Area of Science:
- Analytical Chemistry
- Food Science
- Metabolomics
Background:
- Non-targeted methods (NTMs) offer a comprehensive analytical approach, examining all sample constituents rather than specific targets.
- NTMs are gaining traction in food and feed testing, necessitating clear communication and standardized practices.
- Existing literature often lacks consolidated information on NTM concepts, terminology, and validation.
Purpose of the Study:
- To clarify frequently asked questions regarding terminology associated with non-targeted methods.
- To provide a roadmap and discuss considerations for the validation of NTMs.
- To support the broader adoption and understanding of NTMs in academic research, commercial development, and official control.
Main Methods:
- Literature review and synthesis of current knowledge on NTMs.
- Analysis of common challenges and considerations in NTM application.
- Development of a framework for approaching NTM validation.
Main Results:
- A comprehensive overview of NTM terminology and concepts.
- Identification of key factors influencing NTM validation strategies.
- A structured approach to ensure the fitness-for-purpose of NTMs.
Conclusions:
- Standardized terminology and clear validation approaches are crucial for the effective implementation of NTMs.
- The proposed roadmap facilitates robust NTM validation, enhancing data reliability and comparability.
- NTMs represent a significant advancement in analytical science with broad applicability in food and feed safety.
Keywords:
Food authenticityFood fraudMass spectrometryMethod validationNGSNMRNon-targeted methodsSpectroscopyMore Related Videos
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
22.6K
07:34Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
12.8K
Related Concept Videos
01:15Data Validation
202
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
Key parameters for method validation include:
202
01:23Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
153
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
153
01:21Development of Analytical Methods
501
An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
501
01:18Chromatographic Methods: Terminology
2.3K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
2.3K