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Related Experiment Video

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Automated Sleep Deprivation Setup Using a Shaking Platform in Mice.

Wen-Jie Bian1,2, Luis De Lecea1,2

  • 1Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Bio-Protocol
|February 27, 2023
PubMed
Summary

We developed an automated sleep disruption protocol for mice using a shaking platform. This robust method effectively reduces non-rapid-eye-movement (NREM) and rapid-eye-movement (REM) sleep without causing stress.

Keywords:
AdolescenceAutomated systemMiceShaking platformSleep deprivation

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Sleep Science

Background:

  • The functions of sleep and its role in development are not well understood.
  • Existing sleep deprivation methods present challenges, including stress, inefficiency, and difficulties in application to young animals.

Purpose of the Study:

  • To develop and validate a novel, automated sleep disruption protocol for mice.
  • To assess the effectiveness and robustness of this method in disrupting both NREM and REM sleep without inducing stress.

Main Methods:

  • Utilized a commercially available, shaking platform-based system for automated sleep deprivation in mice.
  • Monitored brain (electroencephalography) and muscle (electromyography) activity to confirm sleep disruption.
  • Applied the protocol to adolescent mice, with validation for adult mice.

Main Results:

  • The automated protocol effectively and robustly reduced both NREM and REM sleep.
  • Sleep deprivation was achieved without causing a significant stress response in the animals.
  • The system operates autonomously, requiring no human supervision.

Conclusions:

  • This automated sleep disruption protocol offers a reliable and less stressful alternative for studying sleep's role in development and function.
  • The method is suitable for both adolescent and adult mice, enhancing its applicability in sleep research.