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Updated: Aug 8, 2025

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Published on: November 10, 2017
Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
Jingjing Zang1, Stephan C F Neuhauss1
1Department of Molecular Life Science, University of Zurich, Zurich, Switzerland.
Abstract:
The zebrafish retina is a canonical vertebrate retina. Since the past few years, with the continually growing genetic toolbox and imaging techniques, zebrafish plays a crucial role in retinal research. This protocol describes a method to quantitatively evaluate the expression of Arrestin3a (Arr3a) and G-protein receptor kinase7a (Grk7a) in the adult zebrafish retina at protein levels by infrared fluorescence western blot. Our protocol can be easily adapted to measure protein levels in additional zebrafish tissues.

