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High GNG4 predicts adverse prognosis for osteosarcoma: Bioinformatics prediction and experimental verification
Xiaohong Jiang1,2, Fuxing Tang3,4, Junlei Zhang5
1Department of Trauma Orthopedic and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Frontiers in Oncology
|February 27, 2023
Summary
Guanine nucleotide binding protein subunit γ 4 (GNG4) is highly expressed in osteosarcoma, acting as an oncogene. High GNG4 expression indicates a poor prognosis and serves as a reliable diagnostic marker for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Guanine nucleotide binding (G) protein subunit γ 4 (GNG4) is implicated in the malignant progression of various tumors.
- The specific role and mechanism of GNG4 in osteosarcoma pathogenesis remain largely uncharacterized.
Purpose of the Study:
- To investigate the biological role of GNG4 in osteosarcoma.
- To determine the prognostic and diagnostic value of GNG4 in osteosarcoma patients.
Main Methods:
- Bioinformatic analysis of public datasets (GSE12865, GSE14359, GSE162454, TARGET) and validation in a clinical cohort.
- Gene Ontology, gene set enrichment, and immune infiltration analyses were performed.
- In vitro experiments were conducted to assess GNG4 function in osteosarcoma cells.
Main Results:
- GNG4 is significantly upregulated in osteosarcoma tissues compared to normal tissues.
- High GNG4 expression is an independent risk factor for poor overall and event-free survival in osteosarcoma patients.
- GNG4 demonstrated high diagnostic accuracy (AUC > 0.9) and, in vitro, silencing GNG4 inhibited cell viability, proliferation, and invasion.
Conclusions:
- GNG4 functions as an oncogene in osteosarcoma.
- GNG4 is a promising biomarker for predicting poor prognosis and a potential therapeutic target in osteosarcoma.
