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Association between PLA2R gene polymorphism and idiopathic membranous nephropathy in Heilongjiang Chinese
Tian Tian1, Jiaxin Zheng1, Yefan Li1
1Department of Nephrology, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China.
Background:
The aim of this study was to investigate the correlation between the phospholipase A2 receptor (PLA2R) gene polymorphism and idiopathic membranous nephropathy (IMN) in Heilongjiang Chinese.
Methods:
Thirty-five patients with IMN confirmed by renal biopsy attending the Heilongjiang Hospital of Traditional Chinese Medicine between June 2021 and December of 2021 were selected as the IMN group, and a group of 25 healthy participants from the Physical Examination Center of Heilongjiang Hospital of Traditional Chinese Medicine were enrolled as healthy controls. Polymerase chain reaction (PCR) was used to identify and genotype 8 single-nucleotide polymorphism (SNP) loci (rs16844715, rs2715918, rs2715928, rs35771982, rs3749119, rs3828323, rs4665143, and rs6757188) of PLA2R and to analyze the PLA2R gene polymorphisms that correlated with IMN. SPSS 26.0 statistical software was used for data analysis, and the chi-squared (χ2) goodness-of-fit test was used to determine whether each SNP genotype and allele in the PLA2R gene complied with the Hardy-Weinberg equilibrium. The qualitative data were analyzed via χ2 or Fisher exact probability method. Logistic regression was used to analyze risk factors, and the odds ratios (ORs) values and 95% confidence intervals (CIs) were calculated. α=0.05 was taken as the test level, and P<0.05 was considered statistically significant.
Results:
Statistically significant differences were found in the genotype and allele frequencies of rs35771982 and rs3749119 between the IMN and control groups (P<0.05). Logistic regression analysis showed that the genotypes rs35771982 GG and rs3749119 CC were associated with IMN susceptibility. Statistically significant differences in uric acid level were found between the rs35771982 GG and CG + CC genotypes (P<0.05), while statistically significant differences in serum albumin were detected between rs3749119 CC and the CT + TT genotypes (P<0.05). Multivariate logistic regression analysis showed that gender, age, and triglyceride levels affected the occurrence of IMN (P<0.05).
Conclusions:
The PLA2R gene polymorphisms rs35771982 and rs3749119 in Heilongjiang Chinese may be related to IMN susceptibility and correlated with clinical indicators of IMN. Gender, age, and triglyceride levels may influence the occurrence of IMN.
Insights
Specific phospholipase A2 receptor (PLA2R) gene variations, rs35771982 and rs3749119, are linked to idiopathic membranous nephropathy (IMN) susceptibility in Heilongjiang Chinese. These PLA2R gene polymorphisms may also correlate with IMN clinical indicators.
Area of Science:
- Genetics
- Nephrology
- Immunology
Background:
- Idiopathic membranous nephropathy (IMN) is a primary cause of nephrotic syndrome in adults.
- The phospholipase A2 receptor (PLA2R) gene is implicated in the pathogenesis of IMN.
- Understanding genetic predispositions is crucial for IMN research.
Purpose of the Study:
- To investigate the association between PLA2R gene polymorphisms and IMN in the Heilongjiang Chinese population.
- To identify specific single-nucleotide polymorphism (SNP) loci within the PLA2R gene that correlate with IMN.
- To explore the relationship between PLA2R gene variants and clinical indicators of IMN.
Main Methods:
- Genotyping of 8 PLA2R SNPs (rs16844715, rs2715918, rs2715928, rs35771982, rs3749119, rs3828323, rs4665143, rs6757188) using Polymerase Chain Reaction (PCR).
- Comparison of genotype and allele frequencies between 35 IMN patients and 25 healthy controls.
- Statistical analysis including chi-squared tests, Fisher's exact test, and logistic regression to determine associations and risk factors.
Main Results:
- Significant differences in genotype and allele frequencies for PLA2R SNPs rs35771982 and rs3749119 were observed between IMN patients and controls (P<0.05).
- The rs35771982 GG genotype and rs3749119 CC genotype were associated with increased IMN susceptibility.
- Associations were found between rs35771982 genotypes and uric acid levels, and between rs3749119 genotypes and serum albumin levels.
Conclusions:
- PLA2R gene polymorphisms rs35771982 and rs3749119 are potentially linked to IMN susceptibility in the Heilongjiang Chinese population.
- These specific PLA2R gene variants may correlate with key clinical indicators of IMN.
- Factors such as gender, age, and triglyceride levels may also influence the development of IMN.
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