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MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

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Mitochondria Transfer in Bone Marrow Hematopoietic Activity.

Abhishek K Singh^1,2, Jose A Cancelas^1,2

¹Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229, USA.

Current Stem Cell Reports

|February 27, 2023

Summary

Bone marrow stromal cells transfer healthy mitochondria to hematopoietic stem cells (HSCs), boosting their function and aiding recovery after transplantation. This mitochondrial transfer is key for blood regeneration and repair.

Keywords:

Gap junction Hematopoietic stem cells Mesenchymal stromal cells Mitochondrial dynamics and transfer Reactive oxygen species Tunneling nanotubes

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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Published on: September 24, 2021

Area of Science:

Stem cell biology
Mitochondrial dynamics
Regenerative medicine

Background:

Hematopoietic stem cells (HSCs) rely on bone marrow (BM) microenvironment crosstalk for homeostasis and regeneration.
Intercellular communication via organelle transfer can repair damaged cells.
Mitochondria are increasingly recognized as regulators of HSC fate.

Purpose of the Study:

To review recent advances in mitochondrial dynamics, focusing on mitochondrial transfer in HSC maintenance.
To explore implications for stem cell transplantation and regenerative medicine.

Main Methods:

Review of current literature on mitochondrial dynamics and transfer in HSCs.
Analysis of experimental evidence regarding intercellular mitochondrial communication.

Main Results:

HSC mitochondrial metabolism is heterogeneous; quiescence and potency depend on mitochondrial dynamics and clearance.
BM stromal cells transfer healthy mitochondria to HSCs, enhancing oxidative phosphorylation and leukocyte expansion.
Mitochondrial transfer from HSCs to BM stromal cells may repair the niche and improve HSC transplantation outcomes.

Conclusions:

Mitochondrial transfer is a critical mechanism for HSC maintenance and regeneration.
Targeting mitochondrial dynamics holds promise for improving stem cell transplantation and regenerative therapies.