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ROS-responsive ADPH nanoparticles for image-guided surgery.

Kangjun Sun1, Ruitong Xu2, Bingyan Xue2

  • 1Nanjing Medical University, Nanjing, China.

Frontiers in Chemistry
|February 27, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel reactive oxygen species (ROS)-responsive fluorescent nanoprobe for enhanced tumor imaging. This probe successfully guided tumor resection in mice, demonstrating its potential for image-guided surgery.

Keywords:
breast cancerimage-guidednanoparticlereactive oxygen speciessurgery

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Area of Science:

  • Biomedical Engineering
  • Chemical Biology
  • Oncology

Background:

  • Organic fluorescent probes with tumor microenvironment (TME)-responsive properties improve tumor imaging signal-to-noise ratio.
  • Existing TME-responsive probes often target pH or GSH, with limited options for high reactive oxygen species (ROS) levels in TME.
  • ROS are crucial biomarkers in the TME, necessitating the development of ROS-specific probes for improved diagnostics and therapeutics.

Purpose of the Study:

  • To develop and evaluate a novel ROS-responsive fluorescent nanoprobe for TME imaging.
  • To investigate the application of this ROS-responsive nanoprobe in image-guided tumor resection.
  • To assess the probe's efficacy in distinguishing tumor sites and reducing background fluorescence.

Main Methods:

  • Preparation of Amplex® Red (ADHP) as an ROS-responsive nanoprobe.
  • In vitro imaging of 4T1 cells to assess ROS responsiveness and real-time imaging capabilities.
  • In vivo fluorescence imaging in 4T1 tumor-bearing mice to evaluate probe performance and background signal reduction.
  • Image-guided tumor resection surgery utilizing the fluorescence signals from the ADHP probe.

Main Results:

  • The ADHP nanoprobe demonstrated excellent ROS response performance.
  • The probe successfully achieved responsive real-time imaging of 4T1 tumor cells by utilizing intracellular ROS.
  • In vivo imaging showed rapid oxidation of ADHP to resorufin in response to ROS, significantly reducing background fluorescence compared to a resorufin-only probe.
  • Successful image-guided resection of 4T1 abdominal tumors was achieved using the ADHP nanoprobe's fluorescence signals.

Conclusions:

  • The developed ADHP nanoprobe is effective for ROS-responsive TME imaging.
  • This ROS-responsive probe shows significant potential for image-guided tumor resection surgery.
  • The study offers a new strategy for designing TME-responsive fluorescent probes for surgical applications.