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Related Experiment Video

Updated: Aug 8, 2025

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Labelling strategies for correlative light electron microscopy.

Hugh Tanner1,2, Olivia Sherwin1, Paul Verkade1

  • 1School of Biochemistry, Biomedical Sciences Building, University of Bristol, University Walk, Bristol, UK.

Microscopy Research and Technique
|February 27, 2023
PubMed
Summary

Correlative light electron microscopy (CLEM) combines techniques for deeper insights. This review explores advanced probes and strategies to overcome visualization challenges in CLEM workflows.

Keywords:
correlative light electron microscopycorrelative microscopymarkersprobes

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Area of Science:

  • Biomedical research
  • Microscopy
  • Cell biology

Background:

  • Imaging techniques like live-cell fluorescence microscopy and electron microscopy (EM) offer complementary information.
  • Correlative Light Electron Microscopy (CLEM) integrates these modalities to enhance sample analysis.
  • Current CLEM workflows face bottlenecks in visualizing specific targets due to probe limitations.

Purpose of the Study:

  • To review recent advancements in probes for CLEM.
  • To provide strategies for selecting appropriate probes for dual-mode visualization.
  • To discuss the advantages and disadvantages of various CLEM probes.

Main Methods:

  • Literature review of CLEM probe development.
  • Analysis of probe characteristics for light and electron microscopy.
  • Discussion of probe selection strategies based on experimental needs.

Main Results:

  • Fluorescence is not directly visible in standard EM, while EM probes like gold particles require specialized light microscopy.
  • Novel probes are being developed to function effectively in both light and electron microscopy.
  • Choosing the right probe is crucial for successful CLEM and overcoming visualization hurdles.

Conclusions:

  • Effective probes are essential for advancing CLEM applications in biomedical research.
  • Selecting dual-modality probes maximizes the benefits of correlative microscopy.
  • Continued development of probes will further enhance CLEM's potential for discovery.