Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.5K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Cell metabolism·2026
Same author

Multilevel Metabolic Engineering of Probiotic <i>Escherichia coli</i> Nissle 1917 for Antibiotic-Free Production of β-Alanine.

ACS synthetic biology·2026
Same author

[Mechanism of moxibustion in inhibiting synovial inflammation of rheumatoid arthritis by regulating macrophage polarization based on the Tim-3/Gal-9 signaling pathway].

Zhen ci yan jiu = Acupuncture research·2026
Same author

A skin-hypothalamus axis couples heat stress and metabolic dysfunction.

Cell·2026
Same author

The Pharmacokinetics and Relative Bioavailability of a Mini-Tablet of Mercaptopurine, a Novel Formulation for Use in Children with Acute Lymphoblastic Leukemia.

Journal of clinical pharmacology·2026
Same author

[Research progress on the mechanism of moxibustion in the treatment of knee osteoarthritis in the past decade].

Zhen ci yan jiu = Acupuncture research·2025

Related Experiment Video

Updated: Aug 8, 2025

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.5K

Chromosome Three-Dimensional Structure Reconstruction: An Iterative ShRec3D Algorithm.

Fang-Zhen Li1, Xue-Fen Zhang2, Hui-Ying Cai1

  • 1School of Computer Science and Technology, Shandong University of Finance and Economics, Jinan, China.

Journal of Computational Biology : a Journal of Computational Molecular Cell Biology
|February 27, 2023
PubMed
Summary
This summary is machine-generated.

An improved iterative ShRec3D algorithm enhances chromosome 3D structure reconstruction from Hi-C data. This universally applicable method boosts ShRec3D performance across various noise and signal conditions.

Keywords:
3D structureHi-CMDSchromosome

More Related Videos

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

409.5K
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.6K

Related Experiment Videos

Last Updated: Aug 8, 2025

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.5K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

409.5K
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.6K

Area of Science:

  • Genomics and Molecular Biology
  • Computational Biology and Bioinformatics

Background:

  • The three-dimensional (3D) structure of chromosomes is crucial for proper genome function, including gene expression, replication, and segregation during mitosis.
  • The development of Hi-C technology since 2009 has spurred significant interest in reconstructing chromosome 3D structures from experimental data.

Purpose of the Study:

  • To present an enhanced iterative version of the ShRec3D algorithm for improved 3D chromosome structure reconstruction.
  • To evaluate the performance and universality of the proposed iterative ShRec3D algorithm.

Main Methods:

  • Development of an iterative algorithm based on the existing ShRec3D method.
  • Application and testing of the iterative ShRec3D algorithm using Hi-C experimental data.
  • Analysis of algorithm performance across diverse data noise and signal coverage ranges.

Main Results:

  • The iterative ShRec3D algorithm significantly enhances the performance of the original ShRec3D method.
  • The improvements achieved by the iterative algorithm are applicable across a wide range of data noise levels.
  • The enhanced algorithm demonstrates broad applicability across various signal coverage ranges, indicating its universality.

Conclusions:

  • The iterative ShRec3D algorithm represents a substantial advancement in 3D chromosome structure reconstruction.
  • The universality of the improved algorithm makes it a valuable tool for analyzing chromosome architecture using Hi-C data.