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Related Experiment Video

Updated: Aug 8, 2025

Real-Time Void Spot Assay
06:39

Real-Time Void Spot Assay

Published on: February 10, 2023

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Real-Time Void Spot Assay.

Marianela G Dalghi1, Nicolas Montalbetti1, Travis B Wheeler2

  • 1Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh.

Journal of Visualized Experiments : Jove
|February 27, 2023
PubMed
Summary

Researchers developed a real-time void spot assay (RT-VSA) to precisely measure mouse voiding behavior. This improved method overcomes limitations of previous assays for better physiological and neurobehavioral studies.

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Area of Science:

  • Neuroscience
  • Urology
  • Animal Behavior

Background:

  • Normal voiding relies on coordinated bladder, urethra, and sphincter function, controlled by the nervous system.
  • The traditional void spot assay (VSA) measures urine spots to study voluntary voiding in mice.
  • Existing VSA methods lack temporal resolution and struggle with quantifying overlapping urine spots.

Purpose of the Study:

  • To develop an improved method for studying voluntary voiding behavior in mouse models.
  • To overcome the limitations of the traditional void spot assay (VSA).
  • To enable precise measurement of voiding frequency, volume, and patterns.

Main Methods:

  • Developed a video-monitored void spot assay, termed real-time VSA (RT-VSA).
  • RT-VSA quantifies voiding frequency, voided volume, and voiding patterns.
  • Measurements can be taken over 6-hour windows during both light and dark phases.

Main Results:

  • The RT-VSA provides enhanced temporal resolution of voiding events.
  • It accurately quantifies voided volume and identifies distinct voiding patterns.
  • The method allows for detailed analysis of mouse micturition over extended periods.

Conclusions:

  • The real-time VSA (RT-VSA) is a significant advancement for studying voluntary micturition.
  • This method offers superior quantification and temporal resolution compared to traditional VSAs.
  • RT-VSA is applicable to diverse mouse-based research on physiological and neurobehavioral aspects of voiding in health and disease.