Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Replication02:40

DNA Replication

50.2K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
50.2K
Replication in Eukaryotes02:31

Replication in Eukaryotes

171.1K
Overview
171.1K
Chromosome Replication02:31

Chromosome Replication

8.8K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.8K
Replication in Prokaryotes01:32

Replication in Prokaryotes

25.1K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
25.1K
The Replisome03:01

The Replisome

34.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
34.1K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

4.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of moderate effect size genes in autism spectrum disorder through a novel gene pairing approach.

Communications biology·2026
Same author

SHANK3-anchored reverse phenotyping identifies a rare-variant-enriched cognitive-motor subgroup of autism.

medRxiv : the preprint server for health sciences·2026
Same author

Genetic architecture of postpartum psychosis: from common to rare genetic variation.

Molecular psychiatry·2026
Same author

Breeding <i>Tm-1</i>-based tomato rootstocks, resistant to tomato brown rugose fruit virus, to impede soil-mediated viral infections.

Frontiers in plant science·2026
Same author

Concomitant Clonal <i>CBFB</i>::<i>MYH11</i> and <i>PDGFRB</i> Fusions in a Case of <i>De Novo</i> Acute Myeloid Leukemia.

Hematology reports·2026
Same author

Cyclin E modulates vulnerability to CDC7 kinase inhibition.

Oncogenesis·2026

Related Experiment Video

Updated: Aug 8, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.5K

The evolution of the human DNA replication timing program.

Alexa N Bracci1, Anissa Dallmann1, Qiliang Ding1

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.

Proceedings of the National Academy of Sciences of the United States of America
|February 27, 2023
PubMed
Summary

DNA replication timing evolves continuously in primates, driven by DNA sequence changes. This ongoing evolution impacts gene regulation and genome stability, particularly in the human lineage.

Keywords:
comparative genomicshuman evolutionreplication timing

More Related Videos

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.0K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.9K

Related Experiment Videos

Last Updated: Aug 8, 2025

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

8.5K
Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

14.0K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

5.9K

Area of Science:

  • Genomics
  • Evolutionary Biology
  • Molecular Biology

Background:

  • DNA replication timing is crucial for gene regulation and genome stability.
  • The evolutionary drivers of replication timing programs in eukaryotes remain largely unexplored.

Purpose of the Study:

  • To investigate the molecular basis and evolutionary consequences of replication timing divergence across primate species.
  • To understand how DNA sequence evolution influences replication timing and gene expression.

Main Methods:

  • Comparative analysis of DNA replication timing across humans, chimpanzees, and rhesus macaques.
  • Identification of genomic regions with significant replication timing variation.
  • Association studies linking replication timing variation with genetic and epigenetic changes.

Main Results:

  • Replication timing patterns closely mirrored primate phylogeny, indicating continuous evolutionary changes.
  • Hundreds of genomic regions showed significant replication timing differences between humans and chimpanzees.
  • Genes in variant regions exhibited correlated changes in expression and chromatin structure.
  • DNA sequence variations were identified as a key driver of interspecies replication timing differences.

Conclusions:

  • Replication timing is under substantial and ongoing evolutionary pressure in the primate lineage, particularly in humans.
  • Sequence alterations are a primary mechanism driving replication timing evolution.
  • Replication timing evolution can influence regulatory evolution and gene expression patterns at specific genomic loci.