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Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
Published on: December 16, 2016
UV-induced microplastics (MPs) aging leads to comprehensive toxicity.
Heyun Yang1, Xiaoliang Li1, MengHan Guo2
1State Key Laboratory of Eco-hydraulics in North west Arid Region, Xi'an University of Technology, Xi'an 710048, China; Department of Municipal and Environmental Engineering, School of Water Resources and Hydro-Electric Engineering, Xi'an University of Technology, Xi'an, Shaanxi 710048, China.
Microplastics (MPs) and their additives pose significant toxicity risks, with some additives contributing to MPs' overall danger. Combining MPs with antibiotics creates complex pollutants with altered toxicity profiles.
Area of Science:
- Environmental Science
- Toxicology
- Microbiology
Background:
- Microplastics (MPs) are prevalent environmental pollutants.
- Additives leaching from MPs during UV-aging can contribute to their toxicity.
- The combined toxicity of MPs and antibiotics is not well understood.
Purpose of the Study:
- To quantitatively assess the toxicity of MPs and their additives.
- To investigate the toxicity of MP-antibiotic complexes.
- To elucidate the combined toxicity mechanisms.
Main Methods:
- Utilized E. coli whole-cell microarray assay to determine the transcriptional effect level index (TELI).
- Evaluated toxicity of four types of MPs and their additives.
- Assessed toxicity of MP-antibiotic mixtures.
Main Results:
- MPs and additives exhibited high toxicity potential (e.g., polystyrene/DEHP).
- Additive leaching significantly contributed to MP toxicity.
- MP-antibiotic complexes showed significantly altered TELI values (e.g., AMX+PVC, CIP+PVC).
- Antibiotics generally decreased PS toxicity but had minor effects on PP and PE.
- Combined toxicity mechanisms varied, including additive, antibiotic, dual, or novel effects.
Conclusions:
- MPs and their leached additives represent a substantial environmental toxicity risk.
- The interaction between MPs and antibiotics results in complex and varied toxicological outcomes.
- Understanding these complex interactions is crucial for environmental risk assessment.

