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Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative

Hayong Jung1, K Kirk Shung1, Hae Gyun Lim2

  • 1Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.

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|February 28, 2023
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Summary
This summary is machine-generated.

Ultrahigh frequency ultrasound transducers achieve high resolution for cellular imaging and manipulation. This technology enables detailed single-cell visualization and mechanical studies, advancing biomedical engineering applications.

Keywords:
B-mode imagingacoustic tweezersingle-cell imagingultrahigh frequency transducerultrasound

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Area of Science:

  • Biomedical Engineering
  • Cellular Imaging
  • Acoustic Microscopy

Background:

  • Ultrasound imaging offers non-invasive tissue visualization.
  • Conventional ultrasound transducers ( < 20 MHz) lack resolution for cellular applications.

Purpose of the Study:

  • To explore ultrahigh frequency (UHF) transducers for advanced biomedical applications.
  • To demonstrate UHF transducer capabilities in cell imaging and acoustic manipulation.

Main Methods:

  • Utilized ultrahigh frequency (UHF) transducers (110 MHz and 410 MHz).
  • Employed an acoustic scanning microscope for imaging.
  • Applied a dual-mode multifunctional UHF transducer for cell trapping and manipulation.

Main Results:

  • Achieved lateral resolutions of 20 μm (110 MHz) and 6.5 μm (410 MHz).
  • Successfully imaged single PC-3 cells and 15 μm beads.
  • Demonstrated single-cell and bead trapping and manipulation using UHF transducers.

Conclusions:

  • UHF transducers significantly enhance resolution for cellular applications.
  • This technology is suitable for single-cell imaging and mechanical studies.
  • UHF transducers show promise for cell deformability and mechanotransduction research.