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Immunohistochemical Expression of Tensin-4/CTEN in Squamous Cell Carcinoma in Dogs
Alexandra Monteiro1,2, Leonor Delgado3,4, Luís Monteiro3,4
1Department of Biology and Environment, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
Abstract:
C-terminal tensin-like (tensin-4/TNS4/CTEN) is the fourth member of the tensin family, frequently described as displaying oncological functions, including cellular migration, invasion, adhesion, growth, metastasis, epithelial to mesenchymal transition, and apoptosis, in several different types of cancer. To investigate, for the first time, the clinical significance of CTEN in squamous cell carcinoma (SCC) of dogs, we studied a total of 45 SCC sections from various dog breeds. The mean age of the affected dogs was 8.9 ± 3.6 years. Immunohistochemistry confirmed strong cytoplasmatic CTEN expression in the basal layer of the epidermis next to the tumor. We detected high CTEN expression associated with the highest grade of the tumor (grade III) and observed 100% of immunopositivity for this tumor grading (p < 0.0001). These data suggest that CTEN is an oncogene in SCC of dogs and a promising biomarker and a therapeutic target for dogs affected by SCC.
Insights
C-terminal tensin-like (CTEN) is linked to cancer progression. High CTEN expression in canine squamous cell carcinoma (SCC) suggests it
Area of Science:
- Oncology
- Veterinary Pathology
- Molecular Biology
Background:
- C-terminal tensin-like (CTEN) protein is implicated in oncological functions across various cancers.
- CTEN's role in canine cancers, specifically squamous cell carcinoma (SCC), remains largely unexplored.
Purpose of the Study:
- To investigate the clinical significance of CTEN expression in canine SCC for the first time.
- To evaluate CTEN as a potential biomarker and therapeutic target in canine SCC.
Main Methods:
- Studied 45 canine SCC tissue sections using immunohistochemistry.
- Assessed CTEN expression in relation to tumor grade and clinicopathological features.
Main Results:
- Confirmed strong cytoplasmic CTEN expression in the epidermis adjacent to canine SCC tumors.
- Found a significant association between high CTEN expression and high-grade (grade III) SCC (p < 0.0001).
- Observed 100% immunopositivity for CTEN in grade III tumors.
Conclusions:
- CTEN functions as an oncogene in canine SCC.
- CTEN represents a promising biomarker and therapeutic target for canine SCC.
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