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Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
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More's the Same-Multiple Hosts Do Not Select for Broader Host Range Phages.

Jupiter Myers1,2, Joshua Davis Ii1, Megan Lollo1,3

  • 1Department of Biology and Toxicology, Ashland University, Ashland, OH 44805, USA.

Viruses
|February 28, 2023
PubMed
Summary

Isolating bacteriophages using multiple hosts does not increase the likelihood of finding broader host range phages. This study found no significant difference in phage host range breadth regardless of isolation method.

Keywords:
bacteriophagehost rangehost-parasite interactionphage isolationphage therapy

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Area of Science:

  • Microbiology
  • Virology
  • Bacteriophage Research

Background:

  • Bacteriophage (phage) host range is crucial for phage therapy applications.
  • Broader host range phages are desirable for targeting diverse pathogen strains.
  • A common assumption is that isolating phages using multiple hosts enhances the discovery of broader host range phages.

Purpose of the Study:

  • To investigate whether isolating phages using multiple hosts increases the likelihood of obtaining broader host range phages.
  • To compare the host ranges of phages isolated using single versus multiple host strategies.
  • To evaluate the efficacy of multiple-host isolation for identifying phages with expanded host ranges.

Main Methods:

  • Utilized a Bacillus cereus group system for phage isolation and characterization.
  • Compared the host ranges of phages isolated using a single host versus four different hosts.
  • Quantified and analyzed the breadth of host ranges for all isolated phages.

Main Results:

  • No significant difference was observed in the breadth of host ranges between phages isolated using one host versus four hosts.
  • Both narrow and broader host range phages were equally likely to be isolated irrespective of the isolation procedure.
  • The study found that multiple hosts during isolation do not consistently yield broader host range phages.

Conclusions:

  • The common belief that multiple-host isolation increases the chances of obtaining broader host range phages is not supported by this study.
  • While other methods like sequential host isolation can yield broader host range phages, multiple-host isolation is not a reliable strategy for this purpose.
  • Alternative methods should be considered for reliably isolating broader host range phages for therapeutic applications.