Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Meeting report of the eight annual Tri-Service Microbiome Consortium Symposium.

BMC proceedings·2025
Same author

Genetic Transformation of the Model Quorum Sensing Bacterium <i>Vibrio campbellii</i> by Electroporation.

Genes·2025
Same author

In Vivo Comparison of Branched vs Linear Pegylation of a Capsule-Degrading Enzyme for Treatment of Anthrax.

ACS omega·2025
Same author

Meeting report of the seventh annual Tri-Service Microbiome Consortium Symposium.

BMC proceedings·2024
Same author

A Quantum-like Model of Interdependence for Embodied Human-Machine Teams: Reviewing the Path to Autonomy Facing Complexity and Uncertainty.

Entropy (Basel, Switzerland)·2023
Same author

Meeting report of the sixth annual tri-service microbiome consortium symposium.

Environmental microbiome·2023

Related Experiment Video

Updated: Aug 8, 2025

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.0K

Automated SSHHPS Analysis Predicts a Potential Host Protein Target Common to Several Neuroinvasive (+)ssRNA Viruses.

Katarina Z Doctor1, Elizabeth Gilmour1, Marilyn Recarte2

  • 1Navy Center for Applied Research in AI (NCARAI) Information Technology Division, U.S. Naval Research Laboratory, Washington, DC 20375, USA.

Viruses
|February 28, 2023
PubMed
Summary

Researchers identified host proteins cleaved by viral proteases, finding ADGRA2 (GPR124) common to nine neuroinvasive viruses. This protein is crucial for blood-brain barrier integrity and angiogenesis.

Keywords:
ADGRA2GPR124SARS-CoV-2SSHHPSWntblood–brain barrierentryhost–pathogenneuroinvasiveproteasesequence-to-symptomsymptom predictionvirus

More Related Videos

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

9.1K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.9K

Related Experiment Videos

Last Updated: Aug 8, 2025

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.0K
Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy
08:51

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

Published on: April 3, 2018

9.1K
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

6.9K

Area of Science:

  • Virology
  • Molecular Biology
  • Neuroscience

Background:

  • Viral genomes contain host-pathogen sequences near protease cleavage sites.
  • Identifying host proteins cleaved by viral proteases is crucial for understanding infection mechanisms.

Purpose of the Study:

  • To identify host proteins cleaved by viral proteases during infection.
  • To develop an automated tool for searching viral protease cleavage sites in the human proteome.

Main Methods:

  • Developed a 'sequence-to-symptom' tool using PHI-BLAST and UniProt.
  • Applied the tool to nine neuroinvasive viruses (VEEV, EEEV, SARS, SARS-CoV-2, MERS, EV-71, JEV, WNV, ZIKV).
  • Analyzed viral protein sequences and human proteome for cleavage sites.

Main Results:

  • Identified ADGRA2 (GPR124) as a common host protein cleaved by proteases of all nine viruses.
  • ADGRA2 is an endothelial regulator essential for blood-brain barrier integrity.
  • Demonstrated cleavage of MYOM1, VWF, DNAH8, ADGRA2, and POT1 by specific viral proteases (SARS-CoV-2 PLpro, MERS PLpro, VEEV nsP2).

Conclusions:

  • Viral proteases cleave critical host proteins, including ADGRA2, impacting blood-brain barrier integrity.
  • The 'sequence-to-symptom' tool effectively identifies host-pathogen interactions.
  • Findings provide insights into neuroinvasive virus pathogenesis and potential therapeutic targets.