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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Related Experiment Video

Updated: Aug 8, 2025

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

CMI: CRISPR/Cas9 Based Efficient Multiplexed Integration in Saccharomyces cerevisiae.

Jie Meng¹, Yue Qiu¹, Yueping Zhang²

¹Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.

ACS Synthetic Biology

|February 28, 2023

Summary

A new CRISPR/Cas9 based multiplexed integration (CMI) system enables quadruple gene integration in yeast. This efficient method simplifies microbial gene expression for industrial production.

Area of Science:

Synthetic biology
Molecular biology
Microbial biotechnology

Keywords:

Brex27 CRISPR/Cas9 S. cerevisiae metabolic engineering multiplexed integration synthetic biology

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