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Related Experiment Video

Updated: Jun 19, 2026

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Published on: January 18, 2022

A new hyperelastic lookup table for RT-DC.

Lucas Daniel Wittwer^1,2,3, Felix Reichel^3,4, Paul Müller³

¹Institute of Numerical Mathematics and Optimisation, TU Freiberg, Akademiestrasse 6, 09599 Freiberg, Germany.

|February 28, 2023

Summary

This summary is machine-generated.

New lookup tables enhance real-time deformability cytometry (RT-DC) analysis by using advanced material models and diverse channel geometries. This improves the accuracy of cell stiffness measurements for researchers.

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Area of Science:

Biophysics
Cell Mechanics
Biomedical Engineering

Background:

Real-time deformability cytometry (RT-DC) quantifies single-cell biophysical properties like stiffness.
Current lookup tables (LUTs) for RT-DC are limited to linear material models and cylindrical geometries.
Accurate cell stiffness extraction is crucial for understanding cell behavior and disease states.

Purpose of the Study:

To develop novel lookup tables (LUTs) for RT-DC analysis.
To incorporate a neo-Hookean hyperelastic material model and non-cylindrical geometries.
To assess the impact of medium rheology and assumptions on cell deformation measurements.

Main Methods:

Finite element method (FEM) simulations were used to derive new LUTs.
Neo-Hookean hyperelastic material model was applied for numerical simulations.
Cell deformation was analyzed in square and cylindrical channel geometries.

Main Results:

Two new LUTs were generated for RT-DC based on neo-Hookean hyperelasticity.
The influence of shear-thinning media on cell deformation was quantified.
Insights into cell strain, stress, and the impact of symmetry assumptions were provided.

Conclusions:

The developed LUTs expand the applicability of RT-DC to more complex material models and geometries.
This work provides a more accurate method for determining cell stiffness from RT-DC data.
Freely available LUTs and numerical cell shapes will advance RT-DC data analysis.