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A therapeutic probe for detecting and inhibiting ONOO- in senescent cells
Ping Huang1, Zihong Li1, Li Nong1
1Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China. weiyinglin2013@163.com.
Abstract:
Aging is an inevitable biological process, characterized by a general decline in the quality of all physiological functions and reactions involving all organs and tissues of the body. Oxidative stress is considered to be the main cause of aging, which may be caused by active nitrogen substances. ONOO- is one of the important active nitrogen substances. Therefore, detecting the changes of ONOO- in senescent cells is of great significance for the study of senescence. However, the study of ONOO- in senescent cells is not deep enough. Here, we designed and synthesized a fluorescent probe FLASN based on flavonol, which integrates ONOO- detection and aging treatment. Our probe FLASN was prepared by a simple synthesis process and was shown to have excellent spectral characteristics. Meanwhile, the results of bioimaging showed that the probe FLASN could detect endogenous/exogenous ONOO- in cells and in vivo, and could reduce the production of ONOO- in cells and in vivo stimulated by metformin. It is worth noting that for the first time, the change of ONOO- in senescent cells and in vivo was detected, and the therapeutic effect of flavonol on senescent cells and in vivo was confirmed, by using the probe FLASN.
Insights
Researchers developed FLASN, a novel fluorescent probe, to detect and treat peroxynitrite (ONOO-) in aging cells. This probe enables the first detection of ONOO- changes in senescent cells and confirms flavonol
Area of Science:
- Biomedical Science
- Chemistry
- Gerontology
Background:
- Aging is linked to oxidative stress, with active nitrogen species like peroxynitrite (ONOO-) playing a key role.
- Understanding ONOO- in senescent cells is crucial for aging research but remains underexplored.
Purpose of the Study:
- To design and synthesize a fluorescent probe for detecting ONOO- in senescent cells.
- To investigate the therapeutic potential of the probe's core structure (flavonol) in aging.
Main Methods:
- Synthesis of a novel flavonol-based fluorescent probe, FLASN.
- Characterization of FLASN's spectral properties.
- Bioimaging studies to detect endogenous/exogenous ONOO- in cells and in vivo.
- Assessment of FLASN's effect on metformin-induced ONOO- production.
Main Results:
- FLASN was successfully synthesized with excellent spectral characteristics.
- The probe effectively detected ONOO- in cellular and in vivo models.
- FLASN demonstrated a reduction in ONOO- production, particularly in metformin-stimulated conditions.
- This study marks the first detection of ONOO- changes in senescent cells and in vivo.
Conclusions:
- FLASN is a valuable tool for detecting ONOO- in aging contexts.
- Flavonol exhibits therapeutic effects on senescent cells and in vivo, evidenced by ONOO- modulation.
- This research provides new insights into the role of ONOO- in aging and potential therapeutic strategies.
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