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Related Experiment Video

Updated: May 5, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Protocol for combining immunological procedures with cell cycle stage synchronization to detect cell-cycle-specific

Toshiyuki Habu¹, Jiyeong Kim¹

¹Department of Food Sciences and Nutrition, School of Food Sciences and Nutrition, Mukogawa Women's University, Nishinomiya, Hyogo 663-8558, Japan.

|February 28, 2023

Summary

This study introduces a method combining co-immunoprecipitation (Co-IP) and immunofluorescence to detect cell-cycle-specific protein complexes. The protocol visualizes protein complex assembly dynamics during the cell cycle.

Keywords:

Cell Biology Cell Culture Cell Separation/Fractionation Microscopy Molecular Biology Protein Biochemistry Signal Transduction

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Related Experiment Videos

Last Updated: May 5, 2026

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Understanding protein complex dynamics is crucial for cell cycle regulation.
Existing methods may lack the precision to capture cell-cycle-specific interactions.

Purpose of the Study:

To present a robust protocol for detecting cell-cycle-specific protein complexes.
To enable visualization of protein complex assembly dynamics.

Main Methods:

Synchronization of cells at specific cell cycle stages using chemical agents.
Preparation of cell extracts and fractionation of protein complexes via density centrifugation.
Co-immunoprecipitation (Co-IP) using specific antibodies followed by immunofluorescence imaging for localization.

Main Results:

Successful detection of cell-cycle-specific protein complexes.
Confirmation of protein-protein interactions through localization studies.
Visualization of the dynamic assembly of protein complexes.

Conclusions:

The combined Co-IP and immunofluorescence protocol provides a powerful tool for studying cell cycle-dependent protein interactions.
This method aids in understanding the temporal regulation of protein complex formation during the cell cycle.