Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil.

Qingqing Tian1, Kunming Xing2, Yongshu Liu1

  • 1Shandong Province Key Laboratory of Detection Technology for Tumor Makers, School of Medicine, Linyi University, Linyi, Shandong 276005, China.

STAR Protocols
|February 28, 2023
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-Route Accessible Zeolites: bridging organic- and inorganic-directed syntheses toward greener zeolite production.

Chemical science·2026
Same author

Spatiotemporal Dynamics of Suitable Habitat for <i>Weigela florida</i>.

Plants (Basel, Switzerland)·2026
Same author

Tertiary Lymphoid Structure: A Potential Therapeutic Target Affecting Immune Response in Autoimmune Diseases.

Clinical reviews in allergy & immunology·2026
Same author

Patient preferences and willingness-to-pay for AI-enabled blended type 2 diabetes care by digital experience and socioeconomic status: a discrete-choice experiment in China.

BMJ open·2026
Same author

Study on GIS-based suitability evaluation of the landscape environment of celebrity former residences in Huanggang.

Scientific reports·2026
Same author

Miniaturized 3D Magnetic Force Sensor via Laser-Assisted Folding and Magnetization for Enhanced Robotic Dexterity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling.

STAR protocols·2026
Same journal

Protocol for generating and culturing high-grade serous ovarian carcinoma organoids from fresh or cryopreserved patient samples.

STAR protocols·2026
Same journal

Protocol for ultrasound-guided injection into the murine portal vein to initiate liver metastasis.

STAR protocols·2026
Same journal

Protocol for semi-automatic quantitative bioimaging analysis of synapse loss.

STAR protocols·2026
Same journal

Protocol for integrated ubiquitination analysis of in vitro E3 ligase-DUB regulation and in vivo ubiquitin chain linkage characterization.

STAR protocols·2026
Same journal

Protocol for constructing multi-ancestry polygenic models using S4-Multi.

STAR protocols·2026
See all related articles

This study introduces a simple, high-throughput single-cell patterning method using ultrathin metal microstencils (UTmS). This technique facilitates cell preparation for downstream applications like calcium release and apoptosis studies.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • High-throughput single-cell preparation is crucial but challenging.
  • Existing methods often lack convenience and efficiency.
  • Advanced microfabrication techniques are needed for precise cell manipulation.

Purpose of the Study:

  • To present a novel protocol for high-throughput single-cell patterning.
  • To demonstrate the utility of reusable ultrathin metal microstencils (UTmS) for cell patterning.
  • To enable downstream analysis of single-cell behaviors.

Main Methods:

  • Design, fabrication, and characterization of UTmS chips.
  • Preparation of substrates and assembly of the UTmS chip.
  • Single-cell patterning and subsequent cell culturing on the chip.
Keywords:
Biotechnology and bioengineeringCell BiologyCell cultureCell isolationHigh Throughput ScreeningSingle Cell

Related Experiment Videos

Main Results:

  • Successful implementation of a simple and high-throughput single-cell patterning workflow.
  • Demonstration of UTmS reusability and effectiveness in cell patterning.
  • Validation of the method for studying single-cell calcium release and apoptosis.

Conclusions:

  • The UTmS-based protocol offers a convenient and efficient solution for high-throughput single-cell patterning.
  • This method provides a valuable tool for various single-cell analyses.
  • The UTmS technology has broad potential in cell-based assays and research.