Related Experiment Video
Updated: Aug 8, 2025
12:35
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
Published on: November 14, 2017
9.5K
Analysis of mitochondrial double-stranded RNAs in human cells
Sujin Kim1, Jimin Yoon1, Keonyong Lee1
1Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
STAR Protocols
|February 28, 2023
Summary
This study details a protocol for analyzing mitochondrial double-stranded RNA (mt-dsRNA) expression. This method helps understand how mt-dsRNAs, released into the cytosol, trigger antiviral immune responses.
Area of Science:
- Molecular Biology
- Immunology
- Cell Biology
Background:
- The human mitochondrial genome is transcribed bidirectionally, producing complementary RNAs.
- These RNAs can form double-stranded RNAs (mt-dsRNAs).
- Cytosolic mt-dsRNAs are known to activate innate antiviral signaling pathways.
Purpose of the Study:
- To present a detailed, adaptable protocol for analyzing mt-dsRNA expression.
- To provide researchers with methods to investigate the role of mt-dsRNAs in cellular responses.
Main Methods:
- The protocol involves three complementary approaches for mt-dsRNA analysis.
- Optimization is described for human cells, with adaptability for other animal cell lines and tissues.
Main Results:
- The study provides a comprehensive methodology for mt-dsRNA detection and quantification.
- The presented protocol facilitates the study of mt-dsRNA's role in innate immunity.
Conclusions:
- This protocol offers a valuable tool for researchers studying mitochondrial RNA biology and antiviral immunity.
- The adaptable nature of the protocol allows for broad application across various biological systems.
