Related Experiment Video
Updated: Aug 8, 2025

Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders
Published on: September 4, 2011
KISS1 Gene Variations and Susceptibility to Idiopathic Recurrent Pregnancy Loss
Fanting Meng1, Aiqin Zhao1, Haiying Lu1
1Department of Obstetrics and Gynecology, Suzhou High Tech Zone People's Hospital, No. 95, Huashan Road, High Tech Zone, Suzhou, 215129, Jiangsu Province, China.
Abstract:
It is widely accepted that kisspeptin plays an integral role in the regulation of reproduction. Genetic variations in the KISS1 gene have been frequently reported to be linked to reproductive diseases, but there is still a lack of data on the association between KISS1 variations and female reproductive disorders. The present study aimed to examine the association of three missense SNPs in the KISS1 gene including rs12998, rs35431622, and rs4889 in association with idiopathic recurrent pregnancy loss (iRPL). A total of 720 individuals were recruited in this study. The DNA from the blood sample was extracted and genotyped using the PCR method. Haplotype and linkage disequilibrium (LD) have also been analyzed. The results of this study suggested that rs12998 G > A and rs4889 C > G had a significant association with iRPL (p < 0.05); while rs35431622 A > G didn't indicate any association with iRPL. A significant association was also found for three haplotypes including C-A-A, G-G-G, and G-G-A in this population. The analysis also showed a significant LD between rs12998 and rs35431622 (P < 0.0005). The rs12998 G > A and rs4889 C > G variants of KISS1 are linked to unexplained recurrent pregnancy loss and may be risk factors for this disease.
Related Concept Videos
Non-LTR Retrotransposons
Meiosis I
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Single Nucleotide Polymorphisms-SNPs
Gene Conversion
Nondisjunction
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...

