Validation of Plateletworks ADP for the ProCyte Dx analyzer
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Published on: November 29, 2024
Matthew R Kornya1, Anthony C G Abrams-Ogg1, Shauna L Blois1
1Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Insights
Platelet function testing in cats using Plateletworks ADP is validated on the IDEXX ProCyte Dx analyzer. This method accurately determines clopidogrel effect and allows sample analysis up to one hour post-collection.
Area of Science:
- Veterinary Hematology
- Platelet Function Assays
- Clinical Pathology
Background:
- Platelet function testing is crucial for assessing clopidogrel's efficacy in cats.
- The Plateletworks assay, which measures platelet aggregation, requires validation on the IDEXX ProCyte Dx analyzer.
- Optimal timing and the utility of other platelet parameters for this assay remain uninvestigated.
Purpose of the Study:
- Validate the Plateletworks ADP assay on the IDEXX ProCyte Dx analyzer.
- Evaluate the usefulness of various platelet parameters with Plateletworks ADP.
- Determine the ideal time frame for sample analysis post-collection.
Main Methods:
- Simultaneous analysis of Plateletworks ADP using ProCyte Dx and ADVIA 2120i analyzers in healthy cats and cats receiving clopidogrel.
- Comparison of complete blood count (CBC) results at different time points between analyzers.
- Evaluation of platelet parameters and aggregation response.
Main Results:
- Platelet aggregation measurements showed significant differences between analyzers (P < .001).
- The Plateletworks ADP assay demonstrated almost perfect agreement (Cohen's kappa) for determining clopidogrel effect.
- No significant changes in platelet parameters or aggregation were observed within one hour of sample collection.
Conclusions:
- The Plateletworks ADP assay is validated for use on the IDEXX ProCyte Dx analyzer in cats.
- Samples for Plateletworks ADP analysis can be processed up to one hour after phlebotomy.
- This validation facilitates accurate platelet function assessment in feline clinical practice.
Background:
Platelet function testing in cats allows determination of clopidogrel effect. Plateletworks assesses aggregation based on decreasing platelet counts on hematology analyzers in response to agonists. It has not been validated for the IDEXX ProCyte Dx analyzer. Ideal time to perform analysis and the utility of other platelet parameters have not been fully assessed.
Objectives:
To validate Plateletworks ADP on the ProCyte Dx, to investigate the utility of various platelet parameters using Plateletworks ADP, and determine the ideal time to perform analysis.
Animals:
Twenty healthy cats recruited from the general population used for transference of reference intervals to a new analyzer, and 10 cats receiving clopidogrel to determine clopidogrel effect.
Methods:
Plateletworks ADP using the ProCyte Dx and ADVIA 2120i analyzer was run simultaneously in both healthy cats and cats receiving clopidogrel, and CBC results at different timepoints were compared between analyzers.
Results:
Aggregation was significantly different (P < .001) between analyzers. Cohen's kappa showed almost perfect agreement for determination of clopidogrel effect, and the area under the curve of the receiver operating characteristic was 1.0. Lower limits of the aggregation reference interval in healthy cats were 28.8% on the ProCyte Dx and 12.5% on the ADVIA 2120i. Coefficients of variation for platelet parameters were not different between analyzers. No significant changes in mean platelet volume, plateletcrit, large platelets, and mean platelet component were identified. No significant change in aggregation was observed within the first hour after phlebotomy.
Conclusions And Clinical Importance:
Our study validated the Plateletworks ADP system on the ProCyte Dx analyzer. Samples may be analyzed up to 1 h after collection.
