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Ultrafast Biomarker Quantification through Reagentless Capacitive Kinetics.

Shaoyu Kang1, Mohamed Sharafeldin1,2, Sophie C Patrick1

  • 1Department of Chemistry, University of Oxford, South Parks Road, Oxford, OX1 3QZ, United Kingdom.

Analytical Chemistry
|March 1, 2023
PubMed
Summary
This summary is machine-generated.

We developed a fast, reagentless method to quantify proteins using redox capacitance. This technique measures binding kinetics in real-time, enabling sensitive protein detection for clinical applications.

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Area of Science:

  • Biomaterials
  • Electrochemistry
  • Analytical Chemistry

Background:

  • Protein quantification is crucial for clinical diagnostics.
  • Existing methods can be time-consuming or require reagents.
  • Need for rapid, sensitive, and reagentless protein detection.

Purpose of the Study:

  • To introduce a facile method for assessing protein binding kinetics.
  • To quantify target proteins using redox capacitance at bioreceptive interfaces.
  • To demonstrate real-time, sensitive protein detection.

Main Methods:

  • Monitoring redox capacitance (Cr) of a receptor-modified conductive polymer interface.
  • Utilizing a continuous flow system for real-time analysis.
  • Applying the method to quantify C-reactive protein (CRP).

Main Results:

  • Capacitance analyses resolved protein association and dissociation in real-time.
  • Electrochemical signal change rate correlated with target concentration.
  • Achieved picomolar level sensitivity for CRP detection in 15 seconds.

Conclusions:

  • The developed methodology offers a rapid and sensitive approach for protein quantification.
  • This reagentless technique is applicable to various clinically relevant targets.
  • Facile assessment of binding kinetics provides a new avenue for biomarker assays.