Vanillin-Catalyzed highly sensitive luminol chemiluminescence and its application in food detection

Among various chemiluminescence (CL) systems, luminol-H₂O₂ system is used extensively due to its cheapness and sensitivity. Herein, 4-hydroxy-3-methoxybenzaldehyde, known as vanillin, was firstly found to be able to catalyze H₂O₂ very efficiently to produce ^•OH and O₂^•-, which can be used to enhance the CL of luminol-H₂O₂ as Co⁺. In alkaline aqueous solution, vanillin catalyzed the dissociation of H₂O₂ into active ^•OH and O₂^•- radicals and accelerated luminol-H₂O₂ reaction to emit strong CL signal. Combining the stabilizing function of β-CD, CL intensity of luminol-H₂O₂ was enhanced further. Thus, dual-signal amplification of luminol-H₂O₂ chemiluminescence based on the catalyzing function of vanillin and the stabilizing function of β-CD was proposed and its mechanism was explored deeply in the manuscript. Interestingly, vanillin is a highly prized flavor compound broadly used as food additive, however, the excessive intake of vanillin is harmful to human and thus the determination of vanillin is very important. On the basis of the luminol-β-CD-H₂O₂/vanillin reaction, a low-cost, rapid and simple CL sensor has been established to detect vanillin. The sensor was able to detect vanillin in the range of 1.0 μM ∼ 75 μM with a detection limit of 0.89 μM (S/N = 3). It can also be used for CL imaging detection with satisfactory results.