Related Experiment Video

Updated: Aug 8, 2025

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

1.8K

Cystathionine-β-synthase X proteins negatively regulate NADPH-thioredoxin reductase C activity

Chau M Tran1, Shoko Mihara1, Keisuke Yoshida1

  • 1Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho 4259-R1, Midori-Ku, Yokohama, 226-8503, Japan.

Biochemical and Biophysical Research Communications
|March 1, 2023
PubMed

Insights

Cystathionine-β-synthase X (CBSX) proteins negatively regulate NADPH-Trx reductase C (NTRC) activity in plants. This interaction, observed in vitro, is dependent on the presence of AMP, revealing a novel regulatory mechanism for redox homeostasis.

Area of Science:

  • Plant biochemistry
  • Redox biology
  • Posttranslational modifications

Background:

  • Redox regulation is crucial for cellular processes, involving modifications of protein thiols.
  • NADPH-Trx reductase C (NTRC) is a key redox regulator in plant chloroplasts, but its activity control is unclear.
  • Cystathionine-β-synthase X (CBSX) proteins are known to interact with NTRC.

Purpose of the Study:

  • To biochemically investigate the functional interaction between CBSX proteins and NTRC from Arabidopsis thaliana.
  • To determine the regulatory role of CBSX proteins on NTRC activity.

Main Methods:

  • In vitro biochemical assays
  • Analysis of protein-protein interactions
  • Enzyme activity assays

Main Results:

  • CBSX proteins were confirmed to interact with NTRC.
  • CBSX proteins were found to inhibit NTRC activity.
  • This negative regulation by CBSX is dependent on the presence of AMP.

Conclusions:

  • CBSX proteins function as negative regulators of NTRC in Arabidopsis thaliana.
  • AMP is essential for the inhibitory effect of CBSX on NTRC.
  • This study elucidates a novel regulatory mechanism controlling NTRC activity and redox homeostasis in plants.
Keywords:
2-Cys PrxCBSX proteinsNTRCRedox regulation

More Related Videos

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

16.3K
Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools05:27

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools

Published on: July 20, 2022

1.9K

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K
Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
12.0K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.8K
Redox Reactions01:27

Redox Reactions

Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
65
The Electron Transport Chain01:30

The Electron Transport Chain

The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
17.1K
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
14.9K