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Related Experiment Videos

Quantitative immunocytochemistry by digital image analysis: application to toxicologic pathology.

G M Levine1, P Brousseau, D J O'Shaughnessy

  • 1Division of Pathology, Bio-Research Laboratories Ltd., Montreal, Canada.

Toxicologic Pathology
|January 1, 1987
PubMed
Summary

This study evaluates antibody-labeled protein localization in tissues using immunocytochemistry and digital image analysis. The method accurately quantifies protein levels, aiding toxicologic pathologists in tissue analysis.

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Area of Science:

  • Immunocytochemistry
  • Digital Image Analysis
  • Toxicologic Pathology

Background:

  • Immunocytochemical techniques enable specific antigen localization in tissue sections.
  • Evaluating antibody-labeled, disease-related proteins requires advanced microscopy and image analysis.

Purpose of the Study:

  • To assess the application of antibody-labeled protein localization using immunocytochemistry and computerized image analysis.
  • To determine the accuracy and objectivity of quantifying protein location and amount in tissues.

Main Methods:

  • Utilized an experimental anti-tumor polyclonal antibody (anti-oncomodulin) for immunocytochemistry on various tissue sections.
  • Employed biotinylated secondary antibodies, avidin-biotin-peroxidase complex (ABC method), and diaminobenzidine visualization.

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  • Performed semi-automatic digital image analysis using a Zeiss/Kontron IBAS I & II system and statistical analysis.

    • Main Results:

    • Successfully localized and determined the optical density of immunolabel in tissue sections.
    • Demonstrated statistically significant differences in immunolabeling between control and experimental groups.
    • Confirmed the ability to objectively measure protein location and relative amounts.

    Conclusions:

    • The combined immunocytochemistry and digital image analysis technique is a powerful tool for toxicologic pathologists.
    • This method allows for accurate and objective determination of protein levels in normal and abnormal tissues.
    • Provides a quantitative approach to immunocytochemical data interpretation.