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LncRNA-BC069792 suppresses tumor progression by targeting KCNQ4 in breast cancer
Yunxiang Zhang1, Xiaotong Dong1, Xiangyu Guo2,3
1Department of Pathology, The First Clinical Medical College of Weifang Medical University, Weifang people's Hospital, Weifang, 261100, China.
Background:
Breast cancer is the most common malignant tumor that threatens women's health. Attention has been paid on the study of long- non-coding RNA (lncRNA) in breast cancer. However, the specific mechanism remains not clear.
Methods:
In this study, we explored the role of lncRNA BC069792 in breast cancer. In vitro and in vivo functional experiments were carried out in cell culture and mouse models. High-throughput next-generation sequencing technology and real-time fluorescence quantitative PCR technology were used to evaluate differentially expressed genes and mRNA expression, Western blot and immunohistochemical staining were used to detect protein expression. RNA immunoprecipitation assay and dual-luciferase activity assay were used to evaluate the competing endogenous RNAs (ceRNA), and rescue and mutation experiments were used for verification.
Results:
We found that lncRNA BC069792 was expressed at a low level in breast cancer tissues, and significantly decreased in breast cancer with high pathological grade, lymph node metastasis and high Ki-67 index groups. Moreover, BC069792 inhibited the proliferation, invasion and metastasis of breast cancer cells in vitro and in vivo. Mechanically, BC069792 acts as a molecular sponge to adsorb hsa-miR-658 and hsa-miR-4739, to up-regulate the protein expression of Potassium Voltage-Gated Channel Q4 (KCNQ4), inhibits the activities of JAK2 and p-AKT, and plays a role in inhibiting breast cancer growth.
Conclusions:
LncRNA BC069792 plays the role of tumor suppressor gene in breast cancer and is a new diagnostic index and therapeutic target in breast cancer.
Insights
Long non-coding RNA BC069792 acts as a tumor suppressor in breast cancer. This study found BC069792 inhibits cancer cell proliferation and metastasis, offering a new diagnostic and therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Breast cancer is a leading cause of mortality in women worldwide.
- Long non-coding RNAs (lncRNAs) are implicated in breast cancer, but their specific roles are not fully understood.
Purpose of the Study:
- To investigate the function and mechanism of lncRNA BC069792 in breast cancer.
- To evaluate BC069792 as a potential diagnostic marker and therapeutic target.
Main Methods:
- Utilized in vitro and in vivo models of breast cancer.
- Employed high-throughput sequencing, RT-qPCR, Western blot, immunohistochemistry, RNA immunoprecipitation, and dual-luciferase assays.
- Conducted rescue and mutation experiments for validation.
Main Results:
- lncRNA BC069792 expression was downregulated in breast cancer tissues, particularly in advanced stages.
- BC069792 suppressed breast cancer cell proliferation, invasion, and metastasis.
- BC069792 functions as a molecular sponge for hsa-miR-658 and hsa-miR-4739, upregulating KCNQ4 and inhibiting JAK2/p-AKT pathways.
Conclusions:
- lncRNA BC069792 acts as a tumor suppressor in breast cancer.
- BC069792 represents a promising diagnostic index and therapeutic target for breast cancer.
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