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Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
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Serum-integrated omics reveal the host response landscape for severe pediatric community-acquired pneumonia

Yi Wang1, Xiaolan Huang2, Fang Li3

  • 1Experimental Research Center, Capital Institute of Pediatrics, Beijing, 100020, People's Republic of China. wildwolf0101@163.com.

Critical Care (London, England)
|March 1, 2023
PubMed

Insights

New biomarkers for severe community-acquired pneumonia (CAP) in children were identified using proteomics and metabolomics. This integrated approach aids in early diagnosis and understanding CAP severity mechanisms.

Area of Science:

  • Pediatric Infectious Diseases
  • Biomarker Discovery
  • Systems Biology

Background:

  • Community-acquired pneumonia (CAP) is a leading cause of mortality in children under five globally.
  • Early diagnosis and understanding the mechanisms of severe CAP are crucial for improving outcomes.

Purpose of the Study:

  • To identify novel early biomarkers for severe CAP.
  • To elucidate the underlying pathological mechanisms contributing to CAP severity in children.

Main Methods:

  • Integrated proteomic and metabolomic analysis of serum samples from severe CAP, non-severe CAP, and healthy control cohorts.
  • Validation of identified biomarkers using ELISA and metabolomics in an independent cohort.
  • Pathway analysis to explore molecular mechanisms of disease severity.

Main Results:

  • Distinct proteomic and metabolic signatures differentiated severe CAP, non-severe CAP, and controls.
  • A novel biomarker panel including C-reactive protein (CRP), lipopolysaccharide (LBP), and three metabolites was developed for CAP identification and severity assessment.
  • Pathway analysis revealed dysregulation in cell death, complement system, coagulation, platelet function, inflammation, glycolysis, and lipid metabolism in severe CAP.

Conclusions:

  • Integrated proteomic and metabolomic analysis offers a promising approach for diagnosing CAP and understanding its severity.
  • The identified biomarkers and pathways provide new insights into the complex pathogenesis of severe CAP in children.
Abstract

Keywords:
Community-acquired pneumoniaDiagnosisHost responseMetabolomicsProteomics

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