[Arrangement in chromatin of DNA sites accessible to Serratia marcescens endonuclease]
Abstract:
Splitting of DNA in rat thymus nuclei by Serratia marcescens endonuclease has been studied. DNA fragments were analyzed by gel electrophoresis. Obtained data indicate that the internucleosomal DNA interacts with histones octamer and is cut by endonuclease to fragments multiple of 10 nucleotides. Limits digestion of nuclei with Serratia endonuclease (up to 50% of DNA acid solubility) leaves in a nondegraded form the chromatin fragments including DNA pieces up to 1000 bases in size-resistant DNA. Partly, the resistant DNA has properties of single-stranded molecules. These data are interpreted so that Serratia endonuclease is able to hydrolyse with some preference one of the DNA strands in chromatin. It can be considered as an evidence of different modes of interaction of the histone core with the two DNA strands.
More Related Videos
11:12Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
07:26Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
Published on: December 26, 2020
Related Concept Videos
Nucleotide Excision Repair
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Homologous Recombination
Single-Strand DNA Binding Proteins
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Mismatch Repair
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
