Related Experiment Video

Updated: Aug 8, 2025

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
07:54

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

8.6K

Multi-omics integration to identify the genetic expression and protein signature of dilated and ischemic

Konstantina Portokallidou1,2, Nikolas Dovrolis1,2, Georgia Ragia1,2

  • 1Laboratory of Pharmacology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.

Frontiers in Cardiovascular Medicine
|March 2, 2023
PubMed

Insights

This study identified gene and protein signatures for dilated cardiomyopathy (DCM) and ischemic cardiomyopathy (ICM). These signatures reveal shared and distinct molecular pathways, offering potential diagnostic biomarkers and therapeutic targets for heart failure (HF).

Area of Science:

  • Cardiology
  • Genomics
  • Proteomics
  • Bioinformatics

Background:

  • Heart failure (HF) is a major cause of morbidity.
  • Dilated cardiomyopathy (DCM) and ischemic cardiomyopathy (ICM) are key HF subtypes.
  • Understanding their molecular signatures is crucial for targeted therapies.

Purpose of the Study:

  • To identify distinct gene expression and protein signatures for DCM and ICM.
  • To characterize the molecular pathways underlying these HF subtypes.
  • To uncover potential diagnostic biomarkers and therapeutic targets for HF.

Main Methods:

  • Utilized omics data from public repositories (GEO and PRIDE).
  • Applied a multilayered bioinformatics approach to analyze transcriptomic and proteomic datasets.
  • Performed enrichment analysis and constructed protein-protein interaction networks.

Main Results:

  • Identified 10 differentially expressed genes/proteins in DCM (DiSig) and 15 in ICM (IsSig).
  • Common pathways include extracellular matrix organization and cellular response to stress.
  • Distinct pathways involve muscle tissue development (DCM) and immune cell activation (ICM).

Conclusions:

  • The study provides molecular insights into HF etiopathology, highlighting similarities and differences between DCM and ICM.
  • The identified DiSig and IsSig signatures offer cross-validated targets at both transcriptomic and proteomic levels.
  • These signatures represent promising novel pharmacological targets and diagnostic biomarkers for HF.
Abstract

Keywords:
dilated cardiomyopathyheart failureischemic cardiomyopathyomics integrationprecision medicineproteomicstranscriptomics

More Related Videos

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

17.5K

Related Concept Videos

Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
15
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
21
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
15
Cardiomyopathy I: Introduction and Classification01:25

Cardiomyopathy I: Introduction and Classification

Cardiomyopathy, or CMP, is a group of diseases affecting the myocardial structure, impairing its ability to pump blood effectively. This condition can lead to arrhythmias, heart failure, or sudden cardiac death.Cardiomyopathies are classified into primary and secondary categories:Primary Cardiomyopathy refers to conditions involving only the heart muscle that are often idiopathic (of unknown cause) or genetic. They primarily affect the myocardium without the involvement of other systemic...
27