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Single-Cell Sequencing Reveals Necroptosis-Related Prognostic Genes of Glioblastoma
Ping Zheng1,2, Dabin Ren1, Yu Cong1
1Department of Neurosurgery, Shanghai Pudong New Area People's Hospital, Shanghai, China.
Background:
Glioblastoma (GBM) is one of the most malignant forms of brain cancer, with the extremely lower survival rate. Necroptosis (NCPS) is also one of the most wide types of cell death, and its clinical importance in GBM is not clear.
Methods:
We first identified necroptotic genes in GBM by single-cell RNA sequencing analysis of our surgical samples and weighted coexpression network analysis (WGNCA) from TCGA GBM data. The cox regression model with least absolute shrinkage and selection operator (LASSO) was used to construct the risk model. Then, KM plot and reactive operation curve (ROC) analysis were used to assess the prediction ability of the model. At last, the infiltrated immune cells and gene mutation profiling were investigated between the high- and low-NCPS groups as well.
Result:
The risk model including ten necroptosis-related genes was identified as an independent risk factor for the outcome. In addition, we found that the risk model is correlated with the infiltrated immune cells and tumor mutation burden in GBM. NDUFB2 is identified to be a risk gene in GBM with bioinformatical analysis and in vitro experiment validation.
Conclusion:
This risk model of necroptosis-related genes might provide clinical evidence for GBM interventions.
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