Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

16S ribosomal RNA modification drives transcript-specific translation efficiency.

bioRxiv : the preprint server for biology·2026
Same author

Machine learning-driven identification of virulence determinants in Borrelia burgdorferi associated with human dissemination.

PLoS computational biology·2026
Same author

Interaction dynamics of borrelia surface proteins with fibronectin.

Biointerphases·2026
Same author

<i>Borrelia mayonii</i> induces carditis but not arthritis in Lyme-susceptible mice.

Frontiers in immunology·2026
Same author

Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi.

PLoS pathogens·2026
Same author

Identification of Previously Unknown DNA-Binding Proteins Using DNA Affinity/Pull-Down Methods Followed by Mass Spectrometry.

Current protocols·2025

Related Experiment Video

Updated: Aug 8, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K

Quantitative analyses of interactions between SpoVG and RNA/DNA.

Timothy C Saylor1, Christina R Savage1, Andrew C Krusenstjerna1

  • 1Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA.

Biorxiv : the Preprint Server for Biology
|March 2, 2023
PubMed
Summary

The Borrelia burgdorferi SpoVG protein binds DNA and RNA. Its highest affinity is for spoVG mRNA, with binding independent of specific sequences or structures.

Keywords:
Borrelia burgdorferiDNA binding proteinElectrophoretic mobility shift assay (EMSA)RNA binding proteinSpoVG

More Related Videos

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.7K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K

Related Experiment Videos

Last Updated: Aug 8, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.7K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • The Borrelia burgdorferi SpoVG protein is a known DNA- and RNA-binding protein.
  • Understanding its interactions with nucleic acids is crucial for elucidating its function.

Approach:

  • Measured and compared binding affinities of SpoVG to various RNAs, single-stranded DNAs (ssDNAs), and double-stranded DNAs (dsDNAs).
  • Focused on the 5' untranslated regions of specific messenger RNAs (mRNAs).
  • Conducted mutagenesis studies on SpoVG RNA and ssDNA sequences and performed binding and competition assays.

Key Points:

  • SpoVG exhibited the highest binding affinity for the 5' end of spoVG mRNA.
  • The lowest observed affinity was for the 5' end of flaB mRNA.
  • Nucleic acid binding by SpoVG is not solely dependent on specific sequences or structures.

Conclusions:

  • SpoVG demonstrates differential binding affinities for various nucleic acids.
  • The binding mechanism of SpoVG to nucleic acids involves both sequence and structural elements, but is not entirely dependent on either.
  • Uracil-to-thymine substitutions in ssDNAs did not alter SpoVG complex formation, suggesting flexibility in binding.