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Updated: Aug 8, 2025
An Epithelial Abrasion Model for Studying Corneal Wound Healing
Published on: December 29, 2021
Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair
Zitong Yu1, Rui Hao1, Xi Chen1
1Bionic Sensing and Intelligence Center, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Abstract:
Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integrated human cornea's barrier effects on the chip. We detail steps to verify the barrier effects and physiological phenotypes of microengineered human cornea. Then, we use the platform to evaluate the corneal epithelial wound repair process. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).1.
