Related Experiment Video
Updated: Aug 8, 2025
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Published on: November 2, 2020
Protocol for in vivo CRISPR screening using selective CRISPR antigen removal lentiviral vectors
Sarah Kate Lane-Reticker1, Emily A Kessler1, Audrey J Muscato1
1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Abstract:
Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR technology in vivo. Here, we present a protocol for genome engineering using selective CRISPR antigen removal (SCAR) lentiviral vectors in Renca mouse model. This protocol describes how to conduct an in vivo genetic screen with a sgRNA library and SCAR vectors that can be applied to different cell lines and contexts. For complete details on the use and execution of this protocol, please refer to Dubrot et al. (2021).1.
