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Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice
Hao Chen1, Jian Liu2, Guo-Ping Shi3
1School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
Abstract:
Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin tolerance test, glucose stimulated insulin secretion (GSIS) assay, and histological analysis for islet number and insulin expression in vivo. We then detail islet isolation, islet GSIS, β-cell proliferation, apoptosis, and programming assays ex vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.