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Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
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Manganese Transporter Proteins in Salmonella enterica serovar Typhimurium

Nakyeong Ha1, Eun-Jin Lee2

  • 1Department of Life Sciences, Korea University, Seoul, 02841, Republic of Korea.

Journal of Microbiology (Seoul, Korea)
|March 2, 2023
PubMed

Insights

Salmonella Typhimurium requires manganese for virulence, utilizing specific transporters for uptake and export. Understanding these manganese transport systems is key to controlling Salmonella pathogenesis.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Metal cofactors are vital for enzyme function.
  • Pathogens acquire essential metal ions for survival, while hosts restrict this access for immunity.
  • Manganese is crucial for Salmonella Typhimurium pathogenesis, aiding survival against stress and influencing metabolism.

Purpose of the Study:

  • To summarize identified manganese transporters in Salmonella Typhimurium.
  • To elucidate the roles of manganese uptake and efflux systems in Salmonella virulence.
  • To highlight the importance of manganese homeostasis for Salmonella survival and growth.

Main Methods:

  • Literature review of identified manganese transporters (MntH, SitABCD, ZupT, MntP, YiiP).
  • Analysis of gene regulation under varying manganese concentrations and stress conditions.
  • Investigation of riboswitch elements and regulatory proteins (MntR, MntS).

Main Results:

  • Three manganese importers (MntH, SitABCD, ZupT) and two exporters (MntP, YiiP) identified in Salmonella.
  • MntH and SitABCD expression is induced by low manganese, oxidative stress, and host NRAMP1.
  • MntP regulation involves MntR and MntS; YiiP regulation requires further study.

Conclusions:

  • Manganese homeostasis is critical for Salmonella Typhimurium virulence.
  • Specific transporters play key roles in manganese uptake and efflux, influencing bacterial survival and pathogenesis.
  • Further research is needed to fully understand the regulation of manganese transport in Salmonella.
Keywords:
Manganese homeostasisMetal cofactorPathogenesisSalmonella enterica serovar TyphimuriumTransporter proteins

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