Identification microenvironment immune features and key genes in elderly stroke patients
Yisheng Peng1, Zhengli Liu2, Guanqi Fu2
1Department of Radiological Intervention, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu, P.R. China.
Medicine
|March 2, 2023
Summary
This study reveals key molecular mechanisms and immune cell changes in elderly stroke patients. Understanding these pathways can improve diagnosis and treatment for stroke in older adults.
Area of Science:
- Neuroscience
- Immunology
- Genomics
Background:
- Stroke affects elderly populations significantly.
- Understanding molecular and immune factors in elderly stroke is crucial.
Purpose of the Study:
- To identify signaling pathways and immune microenvironments in elderly stroke patients.
- To compare molecular differences between young and elderly stroke patients.
Main Methods:
- Downloaded and analyzed transcriptome data (GSE37587).
- Performed differential gene expression analysis, Gene Ontology, and pathway analyses.
- Constructed protein-protein interaction, gene-miRNA, gene-TF, and gene-drug networks.
- Evaluated immune cell infiltration and its correlation with age.
Main Results:
- Identified 240 differentially expressed genes (DEGs), with 222 upregulated.
- Enrichment analysis highlighted viral response, type I interferon signaling, and heme metabolism.
- Ten hub genes were identified, including interferon-related proteins and HLA-G.
- Increased age correlated with higher myeloid-derived suppressor cells and NK T cells, and lower immature dendritic cells.
Conclusions:
- The study provides insights into the molecular mechanisms of stroke in the elderly.
- Identified specific immune cell profiles associated with age in stroke patients.
- Findings can aid in understanding and potentially treating elderly stroke patients.
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